80-20 Development in an Unequal World (7th edn), edited by Tony Daly, Ciara Regan and Colm Regan

Ultrastructural studies on Regan and non-Regan isoenzymes of alkaline phosphatase in human ovarian cancer cells.

cells derived from primary tumors were smeared or sec tioned and stained histochemically for alkaline phosphatase by use of a simultaneous azodye coupling technique with or without amino acid inhibitors (Dand i.-phenylalanine and i.-homoarginine) in the incubation mixtures. These amino acids are able to distinguish the Regan isoenzyme from nonRegan alkaline phosphatases. Kaplow's scoring technique

Regan isoenzyme: a carcinoplacental antigen.

Characterization of Two Hela Sublines: Tcrc-1 Produces Regan Isoenzyme and Tcrc-2, Non-regan Isoenzyme

HeLa Cells have been found to produce a placental form of alkaline phosphatase (1, 8, 17) which is very similar to the Regan isoenzyme, a carcinoplacental antigen found in cancer patients (7-9) . In order to investigate the nature of gene expression of carcinoplacental proteins, HeLa cells were cloned, and a subline (HeLa TCRC-1), monophenotypic for the Regan isoenzyme was selected . The proper...

Regan Iseen/vine in Normal Human Sera

polymerized antibody for 1 hr and subsequently centrifuged. The pellet containing the enzymatically active enzyme-anti body complex is immediately assayed for enzyme activity with 72 mM phenylphosphate as substrate. Control tubes contain ing 0.15 M NaCl instead of inactivated serum were carried out through the procedure to check on the spontaneous hydrolysis of phenylphosphate at alkaline pH va...

Version : 1 Reviewer : Elizabeth Regan A

methods section: In this study, 2177 subjects were included. How many of them were male, and how many of them were female? Comments from the authors: This information has now been added to the abstract. What did the authors mean by multiple adjusted FVC, FEV1 and FEV1/ FVC? Comments from the authors: By multiple adjusted, we mean th...